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Spermidine

SIGMA/S0266 - BioReagent, Molecular Biology, suitable for cell culture, ≥98%

Synonym: 1,8-Diamino-4-azaoctane; N-(3-Aminopropyl)-1,4-diaminobutane

CAS Number: 124-20-9
Empirical Formula (Hill Notation): C7H19N3
Molecular Weight: 145.25
EC Number: 204-689-0
MDL Number: MFCD00008229
Linear Formula: NH2(CH2)3NH(CH2)4NH2
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-S0266-1G 1 g
$65.50
1/EA
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45-S0266-5G 5 g
$225.00
1/EA
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45-S0266-25G 25 g
$879.00
1/EA
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assay ≥98%
density 0.925 g/mL at 25 °C (lit.)
foreign activity DNase, RNase, and protease, none detected
form solid or liquid
grade Molecular Biology
InChI 1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
InChI key ATHGHQPFGPMSJY-UHFFFAOYSA-N
mol wt 145.25 (AMU)
product line BioReagent
Quality Level 200 
refractive index n20/D 1.479 (lit.)
SMILES string NCCCCNCCCN
storage temp. room temp
technique(s) cell culture | mammalian: suitable
Application: Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).
Application: Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.
Biochem/physiol Actions: Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Biochem/physiol Actions: Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Biochem/physiol Actions: Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.
Disclaimer: Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.
General description: Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.
Purity ≥98%
Density 0.925 g/mL at 25 °C (lit.)
Refractive Index n20/D 1.479 (lit.)
Storage Temp. room temp
UNSPSC 12352200

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