Monoclonal Anti-Syntaxin antibody produced in mouse
SIGMA/S0664 - clone HPC-1, ascites fluid
Synonym: Anti-HPC-1; Anti-P35-1; Anti-STX1; Anti-SYN1A
MDL Number: MFCD00164742
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Syntaxin Clone: HPC-1 (Cat. No. S0664). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. SK-N-SH 2. SH-SY5Y 3. U87 4. PC12 5. Neuro-2a 6. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HPC-1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 35 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, bovine, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using monolayer cultures of neonatal retina cells |
| microarray: suitable | |
| western blot: 1:2,000 using crude preparation of synaptic vesicles from rat cerebral cortex | |
| UniProt accession no. | P32850  |
| Application: | Monoclonal anti-syntaxin antibody can be used in immunoprecipitation and immunohistochemistry. It can also be used in western blotting. Further, this product can also be used to label monolayer cultures of neonatal retinal cells and frozen paraformaldehyde fixed tissue sections. |
| Application: | Monoclonal Anti-Syntaxin antibody produced in mouse has been used in: • immunoblotting • immunohistochemistry • immunoprecipitation from hippocampal lysate • immunohistochemical analysis |
| Biochem/physiol Actions: | Syntaxin 1 (STX1A) may control exocytosis in synaptic vesicles. In retina, STX1A may help in the structural formation of the inner nuclear layer (INL) and outer plexiform layer (OPL). Deletion of STX1A in mice affects the functions of hypothalamic-pituitary-ad |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Syntaxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Syntaxin (also cited as HPC-1 antigen), a 35 kDa molecule with carboxyl-terminal membrane anchor is a synaptic protein. Syntaxin 1 is a membrane protein. Neurons express syntaxin 1A. |
| General description: | Syntaxin is a 35kD synaptic protein capable of interacting with the synaptic vesicle protein synaptotagmin. It facilitates the docking and fusion of synaptic vesicles with the plasma membrane. Monoclonal anti-syntaxin antibody can be used to screen λ gt 11 library of rat hippocampus to isolate syntaxin cDNA clones. It can also be used in microarray and immunocytochemistry. Mouse anti-syntaxin antibody reacts specifically with membrane protein syntaxin (35 kD). The product has also shown cross reactivity for rabbit, rat and bovine but not for guinea pig. |
| Immunogen: | synaptosomal plasma-membrane fraction from adult rat hippocampus. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |