Anti-Sortilin antibody produced in rabbit
SIGMA/S0697 - lyophilized powder, affinity isolated antibody
Synonym: Anti-SORT1
Product Type: Chemical
Immunohistochemistry Anti-Sortilin (extracellular): Cat. No. S0697: Immunohistochemical staining of Rat neocortex with Anti-Sortilin (extracellular) antibody. A. Sortilin appears in cortical neurons (red). B. Staining of interneurons with Mouse Anti-Parvalbumin (PV, green). C. Confocal merge of Sortilin and PV demonstrates separate localization in neocortex.
Immunoblotting Anti-Sortilin (extracellular): Cat. No. S0697: Western blot analysis of Mouse (lanes 1,3) and Rat (lanes 2,4) brain lysate. Lanes 1,2. Anti-Sortilin (extracellular) antibody, (1:400). 3,4. Anti-Sortilin (extracellular) antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: 1:200 using rat brain lysates | |
| UniProt accession no. | Q99523  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Sortilin is neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. Sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death |
| Immunogen: | peptide DKDTTRRIHVSTDQD(C) corresponding to residues 320-335 of human sortilin. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.025% sodium azide. |
| Preparation Note: | Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |