Anti-SmB antibody, Mouse Monoclonal
SIGMA/S0698 - clone 12F5, purified from hybridoma cell culture
Synonym: Anti-SNRPB1; Anti-Sm protein B; Anti-Sm-B; Anti-SmB/SmB; Anti-Small nuclear ribonucleoprotein associated protein B; Anti-snRNP-B
Product Type: Chemical
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with Monoclonal Anti-SmB antibody produced in Mouse, Clone: 12F5 (Cat. No. S0698). The antibody was developed using 1:3,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.25 μg/mL antibody 4. 0.125 μg/mL antibody 5. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 12F5, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen ~25 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 0.5-1 μg/mL using HeLa nuclear cell extract | |
| UniProt accession no. | P14678  |
| Application: | • Monoclonal Anti-SmB antibody produced in mouse has been used in: • western blotting • enzyme-linked immunosorbent assay (ELISA) • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | SNRPB (small nuclear ribonucleoprotein-associa |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-SmB (mouse IgG1 isotype) is derived from the hybridoma 12F5-H3.D8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an SmB recombinant protein. |
| General description: | SNRPB (small nuclear ribonucleoprotein-associa |
| Immunogen: | SmB recombinant protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |