SSC Buffer
SIGMA/S0902 - for Northern and Southern blotting, powder blend
Synonym: Saline-sodium citrate buffer
Product Type: Chemical
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| foreign activity | DNAse, none detected |
| Endonuclease, none detected | |
| Exonuclease, none detected | |
| NICKase, none detected | |
| RNAse, none detected | |
| form | powder blend |
| pH | 6.8-7.2 (20-25 °C, 263 mg/L in water) |
| Quality Level | 200  |
| solubility | water: 263 mg/mL, clear, colorless |
| storage temp. | room temp |
| Application: | Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer. |
| Application: | Suitable for: • Preparing nucleic acids for hybridization • Hybridization buffer for Northern and Southern blots • Washing buffer for Northern and Southern blots |
| Features and Benefits: | • Multi-purpose use in hybridizations • Easily reconstituted and diluted to any concentration needed |
| General description: | SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x. |
| Preparation Note: | Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41105319 |