Anti-Superoxide Dismutase (MnSOD) (DD-17)−Cy3 antibody produced in rabbit
SIGMA/S1450 - 1-2 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-IPO-B; Anti-
Product Type: Chemical
Immunofluorescence 3T3 cells were fixed and permeabilized with cold Methanol followed by cold Methanol: Acetone (1:1). Fixed cells were stained with 2 μg/mL Rabbit Anti- MNSOD (DD17), Cy3 conjugate (Cat. No. S1450).
Immunofluorescence HeLa cells were fixed and permeabilized with cold Methanol followed by cold Aceton. Fixed cells were stained with 2 μg/mL Rabbit Anti- MNSOD (DD17), Cy3 conjugate (Cat. No. S1450).
Immunofluorescence NRK cells were fixed and permeabilized with cold Methanol followed by cold Aceton. Fixed cells were stained with 2 μg/mL Rabbit Anti-MNSOD (DD17), Cy3 conjugate (Cat. No. S1450).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1-2 mg/mL |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 2-5 μg/mL using human HeLa, rat NRK and mouse 3T3 cells. |
| UniProt accession no. | P04179  |
| Application: | Anti-Superoxide Dismutase (MnSOD) (DD-17)-Cy3 antibody produced in rabbit has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Superoxide dismutase 2 (SOD2) is an antioxidant enzyme and catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen. The enzyme offers protection against oxidative damage induced by reactive oxygen species and is also radioprotective. The gene is associated with the metastasis and invasion process of salivary adenoid cystic carcinoma. Manganese SOD is involved in detoxification. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The SOD2 (superoxide dismutase 2) gene is mapped to human chromosome 6q25.3. The encoded protein functions as a tetramer and is localized to mitochondria. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 183-199 of human superoxide dismutase (MnSOD), conjugated to KLH. The corresponding sequence is conserved in many eukaryotes. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |