Anti-Sox2 antibody, Mouse monoclonal
SIGMA/S1451 - clone SOX2-6, purified from hybridoma cell culture
Synonym: Anti-ANOP3; Anti-MCOPS3; Anti-SRY (sex determining region Y)-box 2; Anti-SRY-related HMG-box gene 2; Anti-Sex-determining region Y-box 2
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Mouse Anti-SOX2 Clone: SOX2-6 (Cat. No. S1451). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. P19 2. NT2
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SOX2-6, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~35 kDa |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1.0 μg/mL using whole extract of human NT2 cells |
| UniProt accession no. | P48431   |
| Application: | Anti-Sox2 antibody has been used in western blotting and immunohistochemistry. |
| Biochem/physiol Actions: | Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. |
| Biochem/physiol Actions: | SOX2 (sex-determining region Y-box 2) down-regulation reduces the stem cell count, that affects the breast cancer cells in initiating tumor progression. It participates in chemoresistance and regular lung cancer therapies. In mouse, SOX2 plays an important role in branching morphogenesis and regulating lung epithelial cell differentiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-SOX2 (mouse IgG1 isotype) is derived from the hybridoma SOX2-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. |
| General description: | SOX2 (sex-determining region Y-box 2) is a transcription factor that is made of 317 amino acid. It has a HMG (high mobility group box) domain and is a critical transcription regulator of normal stem cell. It is located on human chromosome 3q26.3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |