Anti-Spectrin antibody produced in rabbit
SIGMA/S1515 - whole antiserum
Synonym: Anti-EL2; Anti-HPP; Anti-HS3; Anti-SPH3; Anti-SPTA
MDL Number: MFCD00164739
Product Type: Chemical
Western Blotting Whole extract of Human erythrocytes ghost cells were separated on SDS-PAGE and probed with Anti-Spectrin antibody produced in Rabbit (Cat. No. S1515). The antibody was developed using 1:1,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:400,000 antibody 2. 1:800,000 antibody 3. Negative Control
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:400 |
| UniProt accession no. | P02549  |
| Application: | Anti-Spectrin antibody has been used in western blotting immunofluorescence and immunostaining. |
| Biochem/physiol Actions: | Spectrins modulates the cell morphology and mechanical properties. Spectrins also participates in the cell cycle by controlling the upregulation of membrane receptors. α- and β spectrins are essential for the development of nervous system. |
| Biochem/physiol Actions: | The antibody reacts specifically with the α and β chains of human erythrocyte spectrin. |
| Biochem/physiol Actions: | The spectrin-based membrane skeleton is essential to provide mechanical stability and membrane integrity. It is also responsible for protein organization, trafficking and resilience of erythrocytes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Spectrin is a multifunctional protein that has lipid-binding sites within CH (calponin-homology) tandem domains, a PH (pleckstrin homology) domain and triple helical segments. It is located on chromosome 1q23.1. |
| General description: | Spectrin is the major cytoskeletal component in erythrocytes. It is composed of two types of polypeptides known as α and β with molecular weights of 240 and 220 kDa, respectively. The α- and β-chains form heterotetramers which are linked end-to-end via oligomeric actin and band 4.1 protein. |
| Immunogen: | spectrin from freshly prepared human erythrocyte ghosts. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |