Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human
SIGMA/S1577 - recombinant, expressed in E. coli, lyophilized powder, ≥97% (SDS-PAGE), suitable for cell culture
Synonym: SDF-1; SDF1; SDF-1α; SDF1
Product Type: Chemical
Bioassay EC50 is defined as the effective concentration of SDF-1α/PBSF that elicits a 50% attachment factor in cell based bioassay.
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | ≤1 EU/μg protein Endotoxin |
| packaging | pkg of 10 μg |
| potency | ≤50 ng/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P48061  |
| Biochem/physiol Actions: | SDF-1α functions as a pre-B cell growth factor in the presence of IL-7 (interleukin-7) and a potent chemoattractant for T-lymphocytes and monocytes. It has also been found to be a ligand for CXCR4 (chemokine receptor type 4) or the orphan receptor LESTR/fusin (putative chemokine receptor). By signaling through the receptor, SDF1 may serve as an inhibitor of HIV-1 (human immunodeficiency virus), which utilizes the LESTR/fusin receptor as a point of entry. The SDF-1/CXCR4 signalling pathway is associated with cancer cell proliferation, migration and survival. Overexpression of SDF1 is observed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 offers CXCR4 mediated neuroprotection during post ischemic stroke. |
| General description: | Stromal Cell-Derived Factor 1α (SDF-1α) or pre-B Cell Growth Stimulation Factor (PBSF) is an 8 kDa chemokine peptide. SDF-1 was identified using signal sequence trap cloning. With this method, cDNAs have been cloned using mouse and human stromal cell lines. This cytokine is widely accepted as a CXC chemokine of the intercrine-macrophage inflammatory protein superfamily. The gene has been mapped to chromosome 10q and is encoded by 4 exons. Expression of the human SDF1 gene is abundant in the pancreas, spleen, ovary, and small intestine. The presence of a GC-rich sequence and the lack of a TATA box in the 5∅-flanking region of the gene are consistent with ubiquitous expression. |
| Physical form: | This product is lyophilized from a 0.2 μm-filtered solution containing 10 μg SDF-1α and 0.5 mg bovine serum albumin in PBS. |
| Preparation Note: | Reconstitute the contents of the vial using sterile buffer or medium containing a minimum of 0.1% BSA or HSA to a stock concentration of ≥10 μg/ml. Additional filtration of the stock solution is not recommended as this may result in loss of product due to adsorption onto the filter membrane. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |