Streptavidin−Agarose from Streptomyces avidinii
SIGMA/S1638 - buffered aqueous suspension
Synonym: streptavidin agarose beads; streptavidin agarose resin
MDL Number: MFCD00082035
Product Type: Chemical
| capacity | ≥15 μg/mL binding capacity (biotin) |
| extent of labeling | ≥1 mg per mL |
| form | buffered aqueous suspension |
| matrix | 4% beaded agarose |
| matrix activation | cyanogen bromide |
| matrix attachment | amino |
| matrix spacer | 7 atoms |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| Application: | Streptavidin−agarose from Streptomyces avidinii has been used: • to pull down biotinylated cell surface proteins during the quantification of plasma membrane transforming growth factor β (TGFβ) receptor II (TβRII) and Tβ • RII internalization • in biotinylated miRNA pull-down assay; as secondary antibodies in immunoprecipitation |
| Application: | Streptavidin-agarose is used in protein chromatography, affinity chromatography, and recombinant protein expression and analysis. Streptavidin-agarose has been used to study the oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins. Streptavidin-agarose has also been used to develop a method for screening triplex DNA binders from natural plant extracts. |
| Application: | Used for the purification of biotin containing proteins or DNA binding proteins |
| Biochem/physiol Actions: | Streptavidin is a homotetrameric protein, isolated from Streptomyces avidinii, which, like avidin, has a high affinity for biotin. Streptavidin is slightly anionic (pI ~ 5-6) and non-glycosylated. These properties contribute to its relatively low non-specific binding compared to egg white avidin. Streptavidin is also more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin-agarose can be used to immobilize or isolate various biotinylated macromolecules and complexes (proteins, antibodies, lectins, nucleic acids, receptors, and ligands). The inherent high-affinity streptavidin-biotin interaction requires harsh conditions to release biotinylated macromolecules. This feature makes streptavidin-agarose useful in a variety of affinity purification applications. |
| Physical form: | Suspension in 0.01 M sodium phosphate, pH 7.2, containing 0.05 M NaCl and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |