SOC Medium
SIGMA/S1797 - For use in transformation
Synonym: Super Optimal broth
Product Type: Chemical
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| application(s) | agriculture |
| composition | Dextrose, 3.603 g/L |
| KCl, 0.186 g/L | |
| MgSO4, 4.8 g/L | |
| Tryptone, 20 g/L | |
| Yeast extract, 5 g/L | |
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 500  |
| sterility | 0.2 μm filtered |
| storage temp. | 2-8°C |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest. |
| Features and Benefits: | SOC liquid provides: • Filter-sterilized, ready-to-use format • Convenient package sizes • Standard formulation |
| General description: | Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106200 |