Sialic Acid Aldolase from Escherichia coli K12
SIGMA/S1826 - recombinant, expressed in E. coli BL21, ≥3.0 units/mg protein
Synonym: N-Acetylneuraminate lyase; N-Acetylneuraminate pyruvate-lyase (N-
MDL Number: MFCD00131656
Product Type: Chemical
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| form | lyophilized powder |
| mol wt | 33.4 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli BL21 |
| shipped in | dry ice |
| specific activity | ≥3.0 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 7.5) containing Neu-5-Ac (10 mM) at 37 °C for 15 min and analyzed using capillary electrophoresis with UV detection at 200 nm. |
| Application: | Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes. |
| General description: | Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens. |
| Other Notes: | One unit will catalyze the formation of 1.0 μmol Neu-5-Ac from Man-N-Ac and pyruvate per minute at 37°C at pH 8.0. |
| Physical form: | Lyophilized powder containing Tris-HCl and NaCl |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P261 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 22-26-37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥3.0 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.1.3.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |