α-2,3-Sialyltransferase from Pasteurella multocida
SIGMA/S1951 - recombinant, expressed in E. coli BL21, ≥2 units/mg protein
Synonym: CMP-
MDL Number: MFCD00130470
Product Type: Chemical
One unit will catalyze the formation of 1.0 μmol Neu-5-Ac-α-2,3LacMU from CMP-Neu-5-Ac and Lac-β–OMU per minute at 37 °C at pH 8.0.
α-2,3-Sialyltransferase catalyzes the reaction:CMP-N-acetylneuraminate + β-D-galactosyl-(1→3)-N-acetyl-α-D-galactosaminyl-R = CMP + α-N-acetylneuraminyl-(2→3)-β-D-galactosyl-(1→3)-N-acetyl-α-D-galactosaminyl-R
| form | lyophilized powder |
| mol wt | 46.4 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli BL21 |
| shipped in | dry ice |
| specific activity | ≥2 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Enzymatic activity assays performed in Tris-HCl buffer (100 mM, pH 8.0) containing CMP-Neu-5Ac (1 mM) and Lac-β−OMU (1 mM) at 37°C for 30 min and analyzed using HPLC with a fluorescence detector (excitation at 325 nm and emission at 372 nm). |
| Biochem/physiol Actions: | Sialyltransferase catalyzes the transfer of Neu5Ac from CMP-Neu5Ac as a donor substrate to the β-D-galactosyl-1,4-N-acet |
| Biochem/physiol Actions: | Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides. |
| General description: | Sialyltransferases belongs to the glycosyltransferases family with the capability of catalyzing the transfer of N-acetylneuraminic acid residues. It is a multifunctional enzyme with α-2,3-sialyltransferase activity, α-2,6-sialyltransferase activity, sialidase activity, and trans-sialidase activity. It has a molar mass of 46.4 kDa, with pI, pH being 5.94 and 7.5-8.5 respectively. |
| Other Notes: | One unit will catalyze the formation of 1.0 μmol Neu-5-Ac-α-2,3LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0. |
| Physical form: | Lyophilized powder containing Tris-HCl and NaCl |
| Preparation Note: | Reconstitute the lyophilized powder with a volume of water in the range of 0.1 mL to 1 mL, to give a concentration in the range of 1 unit/mL (1 mL volume of water) to 10 units/mL (0.1 mL volume of water). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.4.99.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |