Staphylococcus aureus
SIGMA/S2014 - buffered aqueous suspension, Wood 46 strain
Product Type: Chemical
| biological source | Staphylococcus aureus |
| composition | Cell suspension, ~10% wet weight/volume |
| form | buffered aqueous suspension |
| Quality Level | 200  |
| sterility | Not processed or packaged aseptically |
| storage temp. | 2-8°C |
| Analysis Note: | This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler. |
| Application: | (Not intended for use as a starter culture.) |
| Application: | Staphylococcus aureus has been used: • to mimic infection and induce fever in Pekin duck • to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva • in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedu |
| Application: | Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies. |
| Biochem/physiol Actions: | Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus. This isolate is useful in understanding protein A role in pathogenesis and virulence. |
| General description: | Staphylococcus aureus Wood 46 is protein A deficient and spa negative. It shares 98% to 99% genome identity with S.aureus and shows a lower surface expression of cell wall-associated protein A. |
| Packaging: | 10, 50 mL in serum bottle |
| Physical form: | Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide |
| Preparation Note: | Produced in pure culture. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |