α-2,6-Sialyltransferase from Photobacterium damsela
SIGMA/S2076 - recombinant, expressed in E. coli BL21, ≥5 units/mg protein
Synonym: β-Galactoside α-2,6-sialyltransferase; CMP-
MDL Number: MFCD00132245
Product Type: Chemical
One unit will catalyze the formation of 1 μmol Neu-5-Ac-α-2,6-LacMU from CMP-Neu-5-Ac and Lac-β–OMU per minute at 37 °C at pH 8.0.
α-2,6-Sialyltransferase catalyzes the reaction: CMP-N-acetylneuraminate + β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosamine = CMP + α-N-acetylneuraminyl-(2→6)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosamine
| form | lyophilized powder |
| mol wt | 56.8 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli BL21 |
| shipped in | dry ice |
| specific activity | ≥5 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.0) containing CMP-Neu-5-Ac (1 mM) and Lac-β−OMU (1 mM) at 37 °C for 30 min and analyzed using HPLC with a fluorescence detector (excitation at 325 nm and emission at 372 nm). |
| Application: | Highly active α2-6 sialyltransferase has been used to prepare high levels of disialylated fragment crystals. |
| Application: | α-2,6-Sialyltransferase from Photobacterium damsela has been used in resialylation and restoration of sialic acids (SAs) in HRT-18G cells. |
| Biochem/physiol Actions: | Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides. |
| Biochem/physiol Actions: | The terminal step of complex N-glycan biosynthesis is catalysed by α-2,6-sialyltransferase (STs). Bacterial α(2,6)-STs possesses broader acceptor substrate specificity when compared to eukaryotic α(2,6)-STs. |
| General description: | Human ST6Gal-I (β-galactoside α-2,6-sialyltransferase 1) is a member of the CAZy family GT29. |
| Other Notes: | One unit will catalyze the formation of 1 μmol Neu-5-Ac-α-2,6-LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0. |
| Physical form: | Supplied as a lyophilized powder containing Tris-HCl and NaCl. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥5 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.4.99.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |