Seppro™ Ammonium Bicarbonate Buffer
SIGMA/S2454
Synonym: ABC buffer; Ammonium hydrogen carbonate; Monoammonium carbonate
Product Type: Chemical
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| application(s) | life science and biopharma |
| color | clear colorless |
| concentration | 2 M |
| form | solution |
| Quality Level | 200  |
| storage temp. | room temp |
| technique(s) | protein expression: suitable |
| Application: | Ammonium bicarbonate buffer has been used as a component of cell lysis/protein extraction buffer for proteomic sample preparation. |
| General description: | Seppro™ Ammonium Bicarbonate Buffer is an effective buffer for protein digestion and deglycosylation reaction. It may be used to coat 2D and 3D electrophoretic gel pieces during digestion of proteins and proteomes. The ammonium bicarbonate buffer also provides moisture during enzymatic cleavage. Ammonium bicarbonate buffer regulates the microenvironmental pH of immobilized cephalosporin C acylase (CCA) for the catalysis of cephalosporin C (CPC) in a batch stirred tank reactor (STR). |
| Legal Information: | 6538 is a trademark of American Type Culture Collection |
| Legal Information: | Seppro is a trademark of Merck KGaA, Darmstadt, Germany |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106500 |