Anti-Soy Protein antibody produced in rabbit
SIGMA/S2519 - fractionated antiserum, buffered aqueous solution
MDL Number: MFCD00162862
Product Type: Chemical
ELISA Anti-Soy Protein antibody produced in Rabbit (Cat. No. S2519), followed by 1:1,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919), was serially diluted to detect Soy Protein by indirect ELISA.
| antibody form | fractionated antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | soy |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: 1:500 |
| indirect ELISA: 1:5,000 |
| Application: | Anti-Soy Protein antibody produced in rabbit has been used in: • enzyme-linked immunosorbent assay (ELISA) • supershift analysis • fluorescent imaging • immunostaining to probe polyvinylene diflouride (PVDF)-bound proteins for cross-reactivity |
| Application: | Anti-Soy Protein antibody produced in rabbit was used for soy quantification in natural rubber latex glove extracts by ELISA at 1:2000 dilution. It was used as a control in supershift analysis of growth-hormone-induced protein binding to lactogenic hormone-responsive region. |
| Biochem/physiol Actions: | Soy protein is a rich source of isoflavones that act as estrogen analogs. They exert both estrogenic and anti-estrogenic effects depending on the tissue in which they act. Isoflavones effect the lipid and bone metabolism and may act as a potential alternative to estrogen in post-menopausal women. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Some cross-reaction is observed with extracts from wheat flour and ovalbumin; no cross-reaction is observed with extracts from maize, rice, potato flour, casein, or meat. |
| General description: | Soy protein in a variety of forms is the basis of many low cholesterol foods and may also be incorporated in animal meat products. |
| General description: | Soy protein is obtained from soybeans. This legume is free of cholesterol and is low in saturated fat. It is mainly used as a replacement for animal proteins in an individual′s diet. This vegetable food has all eight essential amino acids. Soy protein can be used as meat extenders or analogues. |
| Immunogen: | renatured soy protein extracted from soy acetone powder with hot urea. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |