Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse
SIGMA/S2532 - clone SH-B1, ascites fluid
Synonym: Anti-NEF; Anti-S100; Anti-S100-B; Anti-S100beta
MDL Number: MFCD00162834
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Unidirectional photoreceptor-to-Müller glia coupling and unique K+ channel expression in Caiman retina. By: Zayas-Santiago, A., Agte, S., et al. in PLoS One, 2014. PubMed ID: 24831221 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0097155), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoperoxidase staining of human tongue using Monoclonal Anti-S-100 (β-subunit), Clone No. SH-B1 (Cat. No. S2532).
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of Rabbit tongue stained with 1:1000 Monoclonal Anti-S-100 (β-Subunit) antibody produced in Mouse clone SH-B1 (Cat. No. S2532) followed by Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SH-B1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | feline, pig, bovine, sheep, goat, rabbit, canine, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable using denatured-reduced preparations |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using protease-digested sections of human tongue | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| radioimmunoassay: suitable | |
| UniProt accession no. | P04271  |
| Application: | Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse has been used in the following: • ELISA • Immunofluorescence • Immunohistochemistry • Western blotting |
| Biochem/physiol Actions: | Increased expression of S100 is observed in neuronal damage and thus, serves as a biomarker for detecting brain damage and also in several cancers. Also, it is highly permeable through the blood-brain barrier. The gene is known to be associated with cancer progression. |
| Biochem/physiol Actions: | S100β participates in multiple cellular activities including cell growth, cell structure, cell-cell communication, energy metabolism, contraction, and intracellular signal transduction. In utero exposure to cocaine resulting in aberrant S100β levels is linked with neurobehavioral abnormalities and microcephaly. Fetuses with anencephaly show aberrant cell immunoreactivity. Increased levels of this protein are found in plasma of patients with brain damage. S100β is a candidate biochemical marker for spinal cord injury (SCI). Elevated expression of this protein in subpopulations of reactive astrocytes might be linked with hippocampal structural reorganization post-kainic acid (KA)-mediated neurodegeneration. |
| Biochem/physiol Actions: | This antibody recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B1 is independent of Ca2+. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | S100 gene is mapped on human chromosome 21q22.3 and codes for an acidic calcium binding protein B, which weighs 21 kDa. The gene is predominantly expressed in astrocytes, glial cells, Schwann cells and also found in adipose cells, skin, glioblastoma multiforme and melanoma. The protein is localized in extracellular space and cytoplasm. |
| General description: | S100B (S-100β Subunit) belongs to the S100 family of calcium-binding proteins. It is expressed in melanocytes. S-100 is a set of small, thermolabile, highly acidic dimer proteins of approximately 20kDa which are widely distributed in different tissues. Dimeric combinations of two chains, the α-chain (93 amino acids,10.4kDa) and the β-chain (91 amino acids, 10.5kDa), form the three known subtypes of S-100: S-100ao (αα), S-100a (αβ) and S-100b (ββ). Monoclonal Anti-S-100 (β-subunit) (mouse IgG1 isotype) is derived from the SH-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | Bovine brain S-100b |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | The product is provided as ascites fluid containing 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |