Sphingolipid Ceramide N-Deacylase from Pseudomonas sp.
SIGMA/S2563
Synonym: SCDase
MDL Number: MFCD02100603
Product Type: Chemical
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| foreign activity | exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminida |
| protease and sphingomyelinase, essentially free | |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides. It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide. |
| Biochem/physiol Actions: | Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids. |
| Biochem/physiol Actions: | Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids. It condenses fatty acids to sphingosine to generate ceramide. The enzyme mainly acts on neutral and acidic glycosphingolipids. |
| General description: | Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme. |
| Other Notes: | One unit will hydrolyze 1 μmol of asialo GM1 per minute at pH 6.0 at 37 °C. |
| Physical form: | Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352211 |