Anti-S-100 antibody produced in rabbit
SIGMA/S2644 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-42C; Anti-ANX2LG; Anti-ANX2LG; Anti-CAL1L; Anti-CLP11; Anti-Ca[1]; Anti-GP11; Anti-P11; Anti-p10
MDL Number: MFCD00145919
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tongue stained with 20 μg/mL Anti-S-100 antibody produced in Rabbit (Cat. No. S2644) followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of Rat cerebellum stained with 20 μg/mL Anti-S-100 antibody produced in Rabbit (Cat. No. S2644) followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, bovine, guinea pig, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-100 μg/mL using sections of human or rat cerebellum |
| microarray: suitable | |
| UniProt accession no. | P60903  |
| Application: | Anti-S-100 antibody has been used in immunocytochemistry,[25} immunofluorescence and immunostaining. |
| Application: | Anti-S-100 antibody is suitable for use in microarray, and immunohistochemistry (in formalin-fixed, paraffin-embedded sections, or using a conc. of 20-100 μg/mL in sections of human or rat cerebellum). The antibody may also be used in immunoelectrophoresis. |
| Biochem/physiol Actions: | S-100 (S100 calcium binding protein A10) is required for trafficking of TRPV5 and TRPV6 (epithelial Ca2+ channels). S100A10 along with annexin 2, participates in several biological processes such as endocytosis, exocytosis and membrane-cytoskeleton interactions. It controls plasma membrane ion channels and cytosolic phospholipase A2. |
| Biochem/physiol Actions: | S100 calcium binding protein A10 (S-100) plays an important role in depression-like character in rodents. In rat ventricular cardiomyocytes, p11 regulates calcium handling through 5-HT4R pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | S-100 (S100 calcium binding protein A10) belongs to the EF-hand-containing S100 protein family. It is also termed as calpactin light chain, p11 or annexin 2 light chain. This gene is located on human chromosome 1q21. |
| General description: | S100 calcium binding protein A10 (S-100) is located on rat chromosome 2q34. It is also termed as p11. |
| General description: | The S-100 protein family consists of around 16 calcium binding proteins that have distinct tissue specific expression patterns. S-100 proteins are widely expressed in neural and non-neural cell types, as well as in several cancers. These proteins regulate a broad range of cellular functions such as cell growth, signaling, cell structure, and energy metabolism . In immunohistology, the product specifically stains the S-100 protein. The product binds to S-100 protein in human, rat, guinea pig, and bovine. In the brain, the antibody can be used to stain glial and ependymal cells, as well as Schwann cells of the peripheral nervous system. In the skin, melanocytes and Langerhans cells can be stained by the antibody product. In lymph nodes, interdigitating reticulum cells are positively stained by anti-S-100 antibody. S-100 staining can be observed in almost all human benign and malignant melanocytic tumors. |
| Immunogen: | S-100 from bovine brain |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |