Sephacryl®
SIGMA/S300HR - 300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)
Synonym: Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
CAS Number: 65546-95-4
MDL Number: MFCD00165824
Product Type: Chemical
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| compatibility | mode of use size exclusion chromatography |
| description | stability: stable to 0.2M NaOH |
| feature | autoclavable |
| form | suspension (20% aqueous ethanol) |
| matrix | crosslinked allyl dextran/N,N′-methylenebis |
| particle size | 25-75 μm (wet) |
| pore size | 1-400 kDa MW range (dextrans) |
| 10-1500 kDa MW range (globular proteins) | |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans.. |
| Application: | Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine. |
| General description: | • Supplied swollen, suspended in 20% aqueous ethanol • Autoclavable • Stable to 0.2N sodium hydroxide (NaOH) • Store at refrigerated temperature (2-8 °C) |
| General description: | S300HR-750ML′s updated product number is GE17-0599-01 |
| Legal Information: | Sephacryl is a registered trademark of Cytiva |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Risk Statements | 10 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| WGK Germany | WGK 1 |
| Flash Point(F) | 100.4 - 109.4 °F - closed c |
| Flash Point(C) | 38 - 43 °C - closed cup |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151817 |