Sample Buffer, Laemmli 2× Concentrate
SIGMA/S3401
Synonym: SDS Sample Buffer
Product Type: Chemical
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| packaging | vial of 6 mL |
| Quality Level | 100  |
| 200 |
|
| storage temp. | −20°C |
| Application: | Sample Buffer, Laemmli 2× concentrate has been used as a sample buffer for denaturing and loading of protein samples in SDS-PAGE. |
| Biochem/physiol Actions: | Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. |
| Other Notes: | Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. |
| Preparation Note: | Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well. |
| Symbol |    GHS05,GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H311 - H315 - H317 - H318 - H361d - H373 - H412 |
| Precautionary statements | P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | Xn,N |
| Risk Statements | 20/21/22-41-43-48/22-51/53 |
| Safety Statements | 26-36/37/39-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41105323 |