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Streptavidin-R-Phycoerythrin from Streptomyces avidinii

SIGMA/S3402 - buffered aqueous solution

Synonym: Streptavidin-R-PE

MDL Number: MFCD00148522
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-S3402-1ML 1 mL
$131.00
1/EA
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ELISA assay utilizing a biotinylated antibody and Streptavidin-R-Phycoerythrin detection.
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:300 Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 10 μg/mL Streptavidin-R-Phycoerythrin from Streptomyces avidinii (Cat. No. S3402).
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:300 Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 10 μg/mL Streptavidin-R-Phycoerythrin from Streptomyces avidinii (Cat. No. S3402).

 

conjugate phycoerythrin (R-PE) conjugate
form buffered aqueous solution
mol wt ~60 kDa (four identical subunits)
Quality Level 200 
shipped in wet ice
storage temp. 2-8°C
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human appendix or human tonsil tissue
Application: Streptavidin-R-Phycoerythrin along with biotinylated reagents in an avidin/biotin labeling system can be used in flow cytometry.
Application: The streptavidin-phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: Streptavidin is a tetrameric protein having high affinity for biotin. Streptavidin-R-Phycoerythrin is used to stain PNA zip-code microarray to produce a fluorescent signal. The product reacts specifically with antiserum to streptavidin but not with antiserum to avidin.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note: Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352203

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