Streptavidin–β-Galactosidase conjugate

SIGMA/S3887 - streptavidin 1-2 units/mg protein, lyophilized powder

Synonym: Streptavidin–beta-Galactosidase conjugate

MDL Number: MFCD00132385
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-S3887-.5MG	0.5 mg	$422.00 1/EA	Add To Favorites
45-S3887-1MG	1 mg	$773.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

composition	Protein, ≥80% biuret
form	lyophilized powder
Quality Level	200
storage temp.	−20°C
streptavidin activity	1-2 units/mg protein
tag	beta-galactosidase tagged

Application:	Streptavidin–β-Galactosidase conjugate may be used in conjunction with biotin labeled molecules in techniques such as ELISA, immunohistochemistry, protein blotting or with biotinylated DNA probes. It provides a convenient yet highly sensitive and specific detection system. Streptavidin–ß-Galactosidase conjugate has been used in the fusion assay in chinese hamster ovary cells.
Biochem/physiol Actions:	Streptavidin–β-Galactosidase conjugate is reactive with biotin labeled molecules.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	Streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii, is a very high affinity and specificity non-covalent biotin binding protein that is used in a wide range of biochemical/immunological assays such as western blot and ELISAs. It is a component of the streptavidin-biotin system; wherein biotin is conjugated to a target molecule that is later detected by a signal tagged streptavidin. Streptavidin may be covalently tagged with a range of enzymes that develop chromogenic sustrates or fluorescent molecules to support detection systems. β-galactosidase may be used in genetic, molecular and immunologic detection systems. Active β-galatosidase cleaves the detection molecule X-gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) to form an intense blue product that may be detected by chromogenic techniques.
Packaging:	0.5, 1 mg in glass bottle
Physical form:	Contains Tris buffer salts.
Preparation Note:	Streptavidin and β-galactosidase grade VIII are conjugated by a modification of the method of O′Sullivan and purified by gel filtration.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	streptavidin activity: 1-2 units/mg protein
Storage Temp.	−20°C
UNSPSC	12352203

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