Single-strand Binding Protein from Escherichia coli
SIGMA/S3917 - >95% (SDS-PAGE), Molecular Biology, Single strand binding protein to enhance PCR specificity
Synonym: SSB
MDL Number: MFCD00213047
Product Type: Chemical
Representative packaging
| assay | >95% (SDS-PAGE) |
| color | colorless |
| foreign activity | DNase and RNase, none detected |
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| Application: | Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates. |
| Application: | Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays and in protein purification to determine integrity and functionality of the integron attC sites. |
| Biochem/physiol Actions: | Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard. It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication. SSBP mediates the recruitment of other proteins to their sites of function on DNA. |
| General description: | Single-strand Binding Protein is a homotetramer, found in E. coli during the event of DNA metabolism. |
| Physical form: | Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |