Spectinomycin dihydrochloride pentahydrate
SIGMA/S4014 - powder, BioReagent, suitable for cell culture
Synonym: Spectinomycin
CAS Number: 22189-32-8
Empirical Formula (Hill Notation): C14H24N2O7 · 2HCl · 5H2O
Molecular Weight: 495.35
EC Number: 244-554-3
MDL Number: MFCD00150886
Linear Formula: C14H24N2O7 · 2HCl · 5H2O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S4014-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S4014-5G
| antibiotic activity spectrum | Gram-negative bacteria |
| Gram-positive bacteria | |
| application(s) | agriculture |
| biological source | synthetic (inorganic) |
| color | white to pale buff |
| form | powder |
| impurities | endotoxin, tested |
| InChI | 1S/C14H24N2O7.2ClH.5H2O/c |
| InChI key | DCHJOVNPPSBWHK-UXXUFHFZSA |
| mode of action | protein synthesis | interferes |
| potency | ≥603 μg per mg |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | O.O.O.O.O.Cl.Cl.CN[C@@H]1 |
| solubility | H2O: soluble 50 mg/mL at |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.) |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| cell culture | plant: suitable |
| Application: | Spectinomycin dihydrochloride pentahydrate has been used as a supplement in different types of media. |
| Application: | Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L. |
| Biochem/physiol Actions: | Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation. Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenylt Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria. |
| Disclaimer: | Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods. |
| General description: | Chemical structure: aminoglycoside |
| General description: | Spectinomycin has generally been used for the treatment of gonorrhea in males, and a study conducted shows that spectinomycin dihydrochloride pentahydrate provides effective treatment against gonorrhea as well. Clinically, spectinomycin dihydrochloride pentahydrate is easier to use in injections as it shows higher solubility than spectinomycin. |
| Other Notes: | Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place. |
| Packaging: | 5, 25 g in poly bottle |
| Preparation Note: | Stock solutions should be prepared at 10 mg/mL in water and filter sterilized. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |