β-Secretase human
SIGMA/S4195 - recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), ≥10,000 units/mg protein
Synonym: Asp-2; BACE1; Memapsin-2
MDL Number: MFCD03702801
Product Type: Chemical
SDS-PAGE β-Secretase Human (Cat. No. S4195) was assessed on SDS-PAGE.
| assay | ≥90% (SDS-PAGE) |
| description | extracellular domain |
| form | buffered aqueous solution |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells (C-terminal FLAG tagged) |
| shipped in | wet ice |
| specific activity | ≥10,000 units/mg protein |
| storage temp. | 2-8°C |
| UniProt accession no. | P56817  |
| Application: | Human β-secretase or BACE1 has been used to study the binding reaction between amyloid precursor protein and β-secretase. It has also been used in BACE1 assay. |
| Biochem/physiol Actions: | BACE1 (β−Secretase or β−Site APP-Cleaving Enzyme) is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s, therefore BACE1 is a target for inhibitor drug discovery. |
| Biochem/physiol Actions: | BACE1 (β-site APP-cleaving enzyme 1) is a transmembrane aspartyl protease responsible for cleaving the amyloid precursor protein (APP). BACE1-mediated cleavage of amyloid precursor protein (APP) is the first step during the generation of pathogenic amyloid-β peptides, hence making it the major drug target for Alzheimer′s disease (AD). Increased levels of enzymatic activity of BACE1 was found in cerebrospinal fluid from patients having both mild cognitive impairment (MCI) and AD, thereby suggesting the importance of BACE1 enzymatic activity in conversion of MCI to AD. |
| Biochem/physiol Actions: | Transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide. |
| Other Notes: | One unit will hydrolyze 1.0 picomole of 7-methoxycoumarin-4-acety |
| Physical form: | Solution in 20 mM Hepes, pH 7.4, 125 mM NaCl. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥10,000 units/mg protein |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |