Amyloid Precursor Protein β, Secreted human
SIGMA/S4316 - recombinant, expressed in E. coli (N-terminal histidine tagged), solution
Synonym: Amyloid Precursor Protein β, Secreted; sAPPβ
MDL Number: MFCD06201921
Product Type: Chemical
SDS-PAGE Amyloid Precursor Protein β, Secreted human (Cat. No. S4316) was assessed on SDS-PAGE.
| form | solution |
| mol wt | ~100 kDa by SDS-PAGE |
| Quality Level | 200  |
| recombinant | expressed in E. coli (N-terminal histidine tagged) |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P05067  |
| Application: | Human amyloid precursor protein β has been used as peptide standard. |
| Biochem/physiol Actions: | The secreted amyloid precursor β (sAPPβ) is a proteolytic cleavage product of β amyloid precursor protein (APP). APP is cleaved by β-secretase into two fragments, sAPPβ and β-amyloid peptide (Aβ). The Aβ is a component of amyloid plaques, one of the major hallmarks of Alzheimer`s disease (AD), while the sAPPβ is thought to modulate neuronal function and cell survival. Mutation in APP found in Swedish familial AD pedigree increases the susceptibility of APP to β-secretase cleavage and the formation of Aβ and sAPPβ. In contrast, activation of protein kinase C (PKC) decreases the levels of sAPPβ. The trans-Golgi network (TGN) is the major site for β-secretase activity. |
| General description: | Amyloid-β (Aβ) peptides are a major component of the senile plaques characteristic of the Alzheimer brain. It is a type- I transmembrane protein. The gene encoding amyloid-β is localized on human chromosome 21. |
| Packaging: | 25 μg in glass bottle |
| Physical form: | 0.2 μm filtered solution in 20 mM Tris (pH 7.4), 0.5 M NaCl, 1 mM EDTA, 5 mM βME, 2 μg/ml aprotinin |
| Preparation Note: | sAPPβ was expressed in E. coli as a soluble protein and purified under non-denaturing conditions |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |