Streptavidin from Streptomyces avidinii
SIGMA/S4762 - essentially salt-free, lyophilized powder, ≥13 units/mg protein
CAS Number: 9013-20-1
MDL Number: MFCD00082035
Product Type: Chemical
ELISA assay utilizing a biotinylated antibody and streptavidin-peroxidase detection.
HABA Assay HABA is a dye with absorbance and Avidin-binding properties. When HABA is bound to Avidin, the complex absorbs light at 500 nm. When a biotin sample is added, the biotin binds strongly to Avidin and displaces the weakly bound HABA resulting in a decrease in absorbance relative to the amount of HABA displaced.
| application(s) | research pathology |
| capacity | 4 mol/mol (Biotin) |
| form | essentially salt-free, lyophilized powder |
| mol wt | ~60 kDa |
| Quality Level | 200  |
| specific activity | ≥13 units/mg protein |
| storage temp. | −20°C |
| Application: | Streptavidin from Streptomyces avidinii has been used: • in wire functionalization • in streptavidin capture enzyme-linked immunosorbent assay (ELISA) • in microbubble preparation |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Streptavidin derives its name from its bacterial source Streptomyces avidinii and from the hen egg-white protein, avidin, which has high affinity to biotin. Its homologous core shares 33% sequence similarity with avidin, as well as sharing a common tetrameric structure. It is a crystalline tetrameric protein, with a molecular weight of 4*15000Da. It binds four molecules of biotin. Streptavidin lacks carbohydrate and sulfur-containing amino acids. |
| General description: | Streptavidin is a crystalline protein isolated from the bacterium Streptomyces avidinii and possesses biotin-binding ability. It has four binding sites for biotin like avidin and binds four molecules of biotin. Streptavidin is now considered as a new type of biotin-binding protein. |
| Packaging: | 5, 10 mg in glass bottle |
| Preparation Note: | Affinity purified. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥13 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |