Anti-α1-Syntrophin (RA-16) antibody produced in rabbit
SIGMA/S4813 - buffered aqueous solution, IgG fraction of antiserum
Synonym: Anti-SNTA1
MDL Number: MFCD03454688
Product Type: Chemical
Immunohistochemistry Frozen sections, acetone fixed Rat tongue were stained with 1:1,000 Anti-α1-Syntrophin (RA-16) antibody produced in Rabbit (Cat. No. S4813), followed by 1:50 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Western Blotting Rat skeletal muscle homogenate extract were separated on SDS-PAGE and probed with Anti-α1-Syntrophin (RA-16) antibody produced in Rabbit (Cat. No. S4813). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:4,000 antibody 4. 1:2,000 antibody + inhibition with immunizing peptide (5 μg/mL) inhibitor 5. 1:2,000 antibody + inhibition with immunizing peptide (10 μg/mL) 6. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): suitable using 0.5% paraformaldehyde fixed/0.5% Triton X-100 treated rat skeletal muscle |
| microarray: suitable | |
| western blot: 1:2,000 using rat skeletal muscle homogenate extract (membrane fraction) |
| Application: | Anti-α1-Syntrophin (RA-16) antibody produced in rabbit has been used in immunohistochemistry and immunoblotting. |
| Biochem/physiol Actions: | α1-syntrophin (SNTA1) mutations results in LQTS (cardiac channelopathies like long QT syndrome). It modulates the development of adipocyte lipid droplet. It also participates in the survival signaling pathway in myoblasts. α1-syntrophin controls voltage-gated Na+ channels. It acts as a substrate for stress-activated protein kinase-3 (SAPK-3). |
| Biochem/physiol Actions: | α1-Syntrophin recruit signaling proteins(2) to the membrane via association with the dystrophin complex. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α1-syntrophin (SNTA1) is a dystrophin-associated protein, that has four domains- a postsynaptic density protein-95/disc large/zona occludens-1 (PDZ) domain, two pleckstrin homology (PH1 and PH2) domains and a syntrophin unique (SU) domain. It is located on human chromosome 20q11.2. It is usually present in the peripheral cytoplasmic membrane. |
| General description: | Anti-α1-Syntrophin (RA-16) is developed in rabbit using a synthetic peptide corresponding to a sequence of mouse α1-syntrophin The syntrophins are a family of intracellular peripheral membrane proteins (58-60 kDa), comprising at least three isoforms α1, β1, and β2-syntrophins. Syntrophins in mammalian skeletal muscle have been shown to be part of a complex of proteins associated with dystrophin, the product of Duchenne/Becker muscular dystrophy gene. The C-terminal 57 amino acids region of syntrophins is highly conserved among the three isoforms, and may contain the binding site for dystrophin. |
| Immunogen: | synthetic peptide corresponding to a sequence of mouse α1-syntrophin (amino acids 191-206 with N-terminally added lysine) conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |