Anti-SynCAM antibody produced in rabbit
SIGMA/S4945 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-IGSF4; Anti-Necl-2; Anti-RAI75; Anti-SgIGSF; Anti-Tslc1
MDL Number: MFCD07370522
Product Type: Chemical
Immunohistochemistry Frozen sections acetone fixed Rat cerebellum tissue sections were stained with 10 μg/mL Anti-SynCAM antibody produced in Rabbit (Cat. No. S4945), followed by 1:20 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Western Blotting Whole extract of Rat Brain cells were separated on SDS-PAGE and probed with Anti-SynCAM antibody produced in Rabbit (Cat. No. S4945). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable using suitable for IHC on rat brain perfused with 4% paraformaldehyde |
| microarray: suitable | |
| western blot: 0.5-1.0 μg/mL using mouse brain and rat brain extract (S1 fraction) | |
| UniProt accession no. | Q8R5M8  |
| Application: | • Anti-SynCAM antibody produced in rabbit has been used in: • immunoprecipitation • immunoblotting • immunohistochemistry • immunostaining • immunofluorescence |
| Biochem/physiol Actions: | SynCAM is a neurological cell-adhesion protein that regulates the formation of synapses. Expression of synaptic cell adhesion molecule (SynCAM) in non-neuronal cells induces synaptic formation and affects synaptic function in hippocampal neurons. It functions as a homophilic cell adhesion molecule at the synapse. SynCAM is believed to interact with PDZ-domain synaptic proteins CASK and syntenin via its cytoplasmic tail. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-SynCAM is developed in rabbit using a synthetic peptide corresponding to amino acids located at the C-terminus of mouse SynCAM1 conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Synaptic cell adhesion molecule (SynCAM) is a brain-specific protein which has an immunoglobulin domain. It binds to intracellular PDZ-domain protein. SynCAMs have also been called nectin-like molecules (Necls). SynCAM1 is also known as Necl-2/ IGSF4/ RA175/ SgIGSF/ TSLC1. SynCAM contains an N-terminal signal peptide, three extracellular Ig domains, a single transmembrane region and a short C-terminal cytoplasmic domain. It is expressed only in brain and is localized to synapses. |
| General description: | SynCAM proteins consist of a C-terminal cytoplasmic domain, an N-terminal signaling domain and three extracellular immunoglobulin domains.. Anti-SynCAM antibody is specific for SynCAM in mouse and rats. The SynCAM1 immunizing peptide (mouse, amino acids 431-445) specifically inhibits the staining of the SynCAM band in immunoblotting. |
| Immunogen: | synthetic peptide corresponding to amino acids 431-445 located at the C-terminus of mouse SynCAM1 conjugated to KLH. |
| Immunogen: | This immunogen sequence is identical in human SynCAM1, highly conserved (90%) in the splice variant SynCAM2 and displays limited homology (55%) to SynCAM3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |