Anti-Superoxide Dismutase (MnSOD) (DD-17) antibody produced in rabbit
SIGMA/S5069 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-IPO-B; Anti-
MDL Number: MFCD06798545
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluoresence. NRK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 10 μg/mL Anti-Superoxide Dismutase (MnSOD) (DD-17) antibody produced in Rabbit (Cat. No. S5069) (A). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 1:500 Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in Rabbit (Cat. No. S5569) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody. Results Two Anti Superoxide Dismutase (MnSOD) antibodies, S5069 (A) and S5569 (B) target different regions of Superoxide Dismutase (MnSOD) show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin fixed, paraffin-embedded Human heart tissue sections were stained with 10 μg/mL Anti-Superoxide Dismutase (MnSOD) (DD-17) antibody produced in Rabbit (Cat. No. S5069), followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting
Cell extract of human HeLa mitochondria was separated on SDS-PAGE and blotted with 1 μg/mL Rabbit Anti-Superoxide Dismutase (MnSOD) (DD-17) (Cat. No. S5069) and developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
Cell extract of human HeLa mitochondria was separated on SDS-PAGE and blotted with 1 μg/mL Rabbit Anti-Superoxide Dismutase (MnSOD) (DD-17) (Cat. No. S5069) and developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~24 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using biotin/ExtraAvidin®-peroxidase staining of human heart sections |
| indirect immunofluorescence: 10-20 μg/mL using methanol-acetone-fixed rat NRK cells | |
| western blot: 1-2 μg/mL using human HeLa mitochondria extract and a chemiluminescent detection reagent | |
| western blot: 2-4 μg/mL using whole extract of rat brain and a chemiluminescent detection reagent | |
| UniProt accession no. | P04179  |
| Application: | The antibody may be detected by various immunochemical techniques including immunoblotting, co-immunoprecipitation assay (co-IP), immunoprecipitation, indirect immunofluorescence and immunohistochemistry. |
| Biochem/physiol Actions: | Superoxide Dismutase (SOD) catalyzes the dismutation of the cytotoxic superoxide radical (O2 -) to molecular oxygen and hydrogen peroxide. Manganese SOD (MnSOD) has been proposed to function as a tumor suppressor by modulating the activity of redox-sensitive transcription factors and specific signal mediators. MnSOD is involved in detoxification. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Superoxide Dismutase (SOD) is a metalloprotein enzyme. It is widely distributed in the animal and plant kingdoms. The three common mammalian endogenous isozymes are the homodimeric copper-zinc SOD (Cu,ZnSOD, SOD1) found primarily in the cytosol, the homotetrameric glycosylated copper-zinc SOD, extracellular SOD (EC-SOD, SOD3), and the homotetrameric mitochondrial manganese SOD (MnSOD, SOD2). All three are nuclear-encoded. MnSOD is composed of four subunits, each containing one Mn2+. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 183-199 of human superoxide dismutase (MnSOD) with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is conserved in many eukaryotes. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |