Anti-SGK antibody produced in rabbit
SIGMA/S5188 - buffered aqueous solution, IgG fraction of antiserum
Synonym: Anti-SGK
MDL Number: MFCD02686615
Product Type: Chemical
Immunofluorescence
A431 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:200 Rabbit Anti-SGK (Cat. No. S5188). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (Cat. No. 18772). Cells were counterstained with phalloidin rhodamine conjugate to stain Actin (red).
A431 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:200 Rabbit Anti-SGK (Cat. No. S5188). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (Cat. No. 18772). Cells were counterstained with phalloidin rhodamine conjugate to stain Actin (red).
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 1:2,000 Rabbit Anti-SGK (Cat. No. S5188). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. MOLT-4 2. RAJI 3. HepG2 4. A431
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:2,000 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated human epidermal carcinoma A431cell line |
| microarray: suitable | |
| western blot: 1:2,000 using whole cell extract of the human epidermal carcinoma A431 cell line | |
| UniProt accession no. | O00141  |
| Application: | Anti-SGK antibody produced in rabbit has been used in : • immunoprecipitation • western blotting • immunofluorescence |
| Biochem/physiol Actions: | SGK (serum and glucocorticoid-regulated protein kinase) plays an important role in signal transduction activated by mineral corticoids. It is also regulated at the transcription level by stimuli including glucocorticoid, serum, follicle stimulating hormone as well as changes in cell volume. SGK levels are strongly affected by osmotic changes. SGK is strongly and rapidly stimulated in kidney cells in response to aldosterone and stimulates epithelial Na+ channels, suggesting a central role of SGK in the regulation of sodium transport and homeostasis. SGK transcription is markedly increased in diabetic nephropathy, in response to excessive extracellular glucose concentrations. The localization of SGK in the cell is regulated by hormones or serum. For cell cycle progression, the shuttling of SGK between nucleus and cytoplasm is required. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-SGK is developed in rabbit using a synthetic peptide corresponding to the C-terminus of human SGK conjugated to KLH (keyhole limpet hemocyanin). SGK is localized to the perinuclear or cytoplasmic compartment as a hypophosphorylated protein. |
| General description: | SGK (serum and glucocorticoid-regulated protein kinase) is a 50 kDa member of Ser/Thr kinase family. Sequence of sgk is well conserved across species, the human and rat SGK amino acid sequences are 96-98% identical. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |