Anti-Sirt1 antibody, Mouse monoclonal
SIGMA/S5196 - clone SIR11, purified from hybridoma cell culture
Synonym: Anti-SIRTUIN1; Anti-Sir2α; Anti-mouse homologue of yeast Sir2
MDL Number: MFCD08705602
Product Type: Chemical
Immunofluorescence C2C12 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Monoclonal Anti-Sirt1 Clone: SIR11 (Cat. No. S5196). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Western Blotting Whole extract of C2 Mouse myoblast cells were separated on SDS-PAGE and probed with Anti-Sirt1 antibody, Mouse monoclonal, Clone: SIR11 (Cat. No. S5196). The antibody was developed using 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.05 μg/mL antibody 2. 0.1 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| purified immunoglobulin | |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SIR11, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~105 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract of C-2 cells | |
| UniProt accession no. | Q923E4  |
| Application: | Anti-Sirt1 antibody, Mouse monoclonal has been used in: • western blot analysis • enzyme-linked immunosorbent assay (ELISA) • immunohistochemical methods |
| Application: | Monoclonal Anti-Sirt1 antibody is suitable for use in western blot (1-2 μg/mL using total cell extract of C-2 cells), immunoblot (approx. 105 kDa), microarray and indirect ELISA. |
| Biochem/physiol Actions: | Monoclonal Anti-Sirt1 (Sir2α) antibody is specific for Sir2α in mice. |
| Biochem/physiol Actions: | SIRT1 is a highly conserved sirtuin family deactelyase that modulates heart metabolism . SIRT1 has also been implicated in senescence and aging . Thus, SIRT1 may be used as a target for treating age-related diseases. |
| Biochem/physiol Actions: | Sirt1 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes O-acetyl-ADP-ribose. Through histone deacetylation, Sirt1 may silence chromatin. It appears that Sirt1 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction. Sirtuin 1 (SIRT1) plays an important role in neuronal proliferation and vascular cells. It also plays an athero-protective role. Mutations in the SIRT1 gene affects the metabolism, cholesterol and fat deposition. SIRT1 has the ability to intermediate the insulin resistance through an inflammatory process, reactive oxygen species, gluconeogenesis and adiponectin level. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Sirt1 (Sir2) (mouse IgG1 isotype) is derived from the hybridoma SIR11 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids at the C-terminus of mouse Sir2 with C-terminal added lysine. Sirt proteins are a family of proteins that are found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity. Sirtuin 1 (SIRT1) protein, a mammalian homolog of yeast Sir2, is a NAD-dependent histone deacetylase. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |