Sericin Bombyx mori (silkworm)
SIGMA/S5201
Synonym: Bombyx mori Extract; Sericin Protein
Product Type: Chemical
P3U1 myeloma cells (1×106) in 1 mL of freezing medium were frozen at -80 °C for 1 day. The cryovials were then thawed and the viability was determined. Freezing medium 1: FBS supplemented with 10% DMSO; freezing medium 2: freezing medium 3 supplemented with 1% sericin; freezing medium 3: PBS supplemented with 0.5% maltose, 0.3% proline, 0.3% glutamine and 10% DMSO; freezing medium 4: PBS supplemented with 10% DMSO. The error bars indicate S.D. (n=3). *P<0.05.
P3U1 myeloma cells 1×106 in 1 mL of freezing medium were frozen at -80 °C for 1 day. The vials were then thawed and the viability of the cells was determined immediately. Freezing medium 1: FBS upplemented with 10% DMSO; freezing medium 2: PBS supplemented with 1% sericin, 0.5% maltose, 0.3% proline, 0.3% glutamine and 10% DMSO; freezing medium 3: Competitor A 4: Competitor B 5: Competitor C; 6: PBS supplemented with 10% DMSO. The error bars indicate S.D. (n=3). *P<0.05.
Hybridoma cells (1×106) in 1 mL of freezing medium were frozen at -80 °C for 1 day. The vials were then thawed and then the vials were frozen and thawed again before the viability was determined. Freezing medium 1: FBS supplemented with 10% DMSO; freezing medium 2: PBS supplemented with 1% sericin, 0.5% maltose, 0.3% proline, 0.3% glutamine and 10% DMSO; freezing medium 3: Competitor A 4: Competitor B 5: Competitor C; 6: PBS supplemented with 10% DMSO. The error bars indicate S.D. (n=3). *P<0.05.
CHO cells (1×106) in 1 mL of freezing medium were frozen at -80 °C for 1 day. The vials were then thawed and the viability of the cells was determined immediately. Freezing medium 1: FBS supplemented with 10% DMSO; freezing medium 2: PBS supplemented with 1% sericin, 0.5% maltose, 0.3% proline, 0.3% glutamine and 10% DMSO; freezing medium 3: Competitor A 4: Competitor B 5: Competitor C; 6: PBS supplemented with 10% DMSO. The error bars indicate S.D. (n=3). *P<0.05.
PC12, NHDF, HEK or insect Sf-9 cells (1×106) in 1 mL of freezing medium were frozen at -80° C for 1 day. The vials were then thawed and the viability of the cells was determined. Freezing media: FBS supplemented with 10% DMSO (a); PBS supplemented with 1% sericin, 0.5% maltose, 0.3% proline, 0.3% glutamine and 10% DMSO (b); and PBS supplemented with 10% DMSO (c). The error bars indicate S.D. (n=3). *P<0.05.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S5201-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S5201-5G
| form | powder |
| impurities | ≤1 EU/mL endotoxin |
| ≤100 CFU/g total microbial count (Bacteria) | |
| ≤100 CFU/g total yeast and mold (Fungi) | |
| Quality Level | 200  |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | A8CEQ1  |
| D2WL76 |
| Application: | Sericin Bombyx mori (silkworm) has been used: • as a standard to evaluate the physicochemical and biological characteristics of the crude sericin extracts • as a growth medium supplement to culture to study its effect on the production of infectious Zika virus (ZIKV) particles by C6/36 and Vero cells, compared to serum-free cultures and cultures supplemented with 5% fetal bovine serum (FBS) • in the development and optimization of a bio-origami film for skin regeneration |
| Biochem/physiol Actions: | Sericin acts as a gum binder to maintain the intact structure of the cocoon. The strong polar side groups of sericin affect its physical and biological properties. Studies show that sericin exhibits various biological functions such as antioxidant and antityrosinase activities, collagen production that is essential in wound healing, anti-inflammatory, antitumoral, anti-aging, and antimicrobial actions. It is known to promote stability and prolong the release of controlled drug-releasing biomaterials. Sericin has several beneficial characteristics for culturing mammalian cells. |
| Features and Benefits: | • Sericin has cryoprotective properties that allow for replacement of fetal bovine serum (FBS) in cryopreservation media. • 1% sericin (w/v) along with 0.5% (w/v) maltose, 0.3% (w/v) proline, 0.3% (w/v) glutamine and 10% dimethyl sulfoxide (DMSO) is comparable to 90% FBS and 10% DMSO. • Sericin can act as a FBS replacement in cell culture and stimulate cell growth. • In certain situations, it can inhibit cell death. |
| General description: | Sericin, a water-soluble globular glycoprotein, is derived from the silkworm Bombyx mori. It is rich in serine, aspartic acid, and glycine. Sericin is localized around the fibroin fibers with successive sticky layers, to form the cocoon. It constitutes 20%-30% of the total cocoon mass. Sericin is composed of 18 types of amino acids of which most of them contain strong polar (hydroxyl, carboxyl, and amino) side groups. |
| Packaging: | 1, 5 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352202 |