Streptolysin O from Streptococcus pyogenes
SIGMA/S5265 - lyophilized powder, Protein ~3 % by Lowry, 25,000-50,000 U/vial
CAS Number: 98072-47-0
EC Number: 308-500-3
MDL Number: MFCD00132389
Product Type: Chemical
Streptolysin O is an antigenic protein capable of forming pore complexes in mammalian cells. The monomer molecular weight is reported to be 64–69 kDa. Pore complexes are reported to be up to 30 nm in diameter and appear to be comprised of a double wall containing as many as 125 monomers.
SDS-PAGE Streptolysin O from Streptococcus pyogenes (Cat. No. S5265) was assessed on SDS-PAGE.
| antibiotic activity spectrum | neoplastics |
| color | white |
| composition | Protein, ~3% Lowry |
| form | lyophilized powder |
| mode of action | cell membrane | interferes |
| mol wt | native 69 kDa |
| Quality Level | 200  |
| solubility | H2O: soluble 1 mg/mL |
| specific activity | 25,000-50,000 U/vial |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.) |
| storage temp. | 2-8°C |
| Application: | Permeabilizes membranes to permit cellular uptake of large or charged molecules. |
| Application: | Streptolysin O is a toxin secreted by Streptococcus pyogenes and is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery. It is a potential anticancer agent and is used to study suicide cancer gene therapy. |
| Application: | Used to create holes (reversible pores) in cholesterol-enriched plasma membranes of eukaryotic cells allowing selective permeabilization with molecules such as proteins (up to 100 kDa) and siRNAs. |
| Biochem/physiol Actions: | Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membrane to make a large pore (25 to 30 nm), which DNA, RNA, peptides and proteins may pass. It is thiol-activated. It is inhibited by allicin, an active component of garlic. |
| Biochem/physiol Actions: | Thiol-activated toxin that permeabilizes animal cell membranes. The protein binds as a monomer to membrane cholesterol and subsequently polymerizes into large arc- and ring-shaped structures surrounding pores of >12 nm. |
| Biochem/physiol Actions: | Thiol-activated toxin that permeabilizes animal cell membranes.. |
| Other Notes: | Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place |
| Other Notes: | One unit will cause 50% lysis of 2% red blood cell suspensions in phosphate buffered saline, pH 7.4, after incubation at 37 °C for 30 min. |
| Packaging: | 25KU |
| Physical form: | Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA. |
| Symbol |    GHS05,GHS06,GHS09 |
| Signal word | Danger |
| Hazard statements | H300 + H310 + H330 - H315 - H318 - H411 |
| Precautionary statements | P260 - P262 - P273 - P280 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310 - P305 + P351 |
| RIDADR | UN 2811PIH 6.1 / PGI |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 25,000-50,000 U/vial |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |