Anti-Sir2 (AS-16) antibody produced in rabbit
SIGMA/S5313 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-SIR2; Anti-SIR2L1; Anti-SIR2alpha
MDL Number: MFCD03792039
Product Type: Chemical
Immunofluorescence NIH3T3 cells were fixed and stained with 20 μg/mL Anti-Sir2 (Cat. No. S5313). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate. Nuclear staining with DAPI.
Immunofluorescence HeLa cells were fixed and stained with 20 μg/mL Anti-Sir2 (Cat. No. S5313). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate. Nuclear staining with DAPI.
Immunoblotting Nuclear extract of HeLa (lanes 1-2) and lysate of Hct-116 (lanes 3-4) cells were separated on SDS-PAGE, blotted with Anti-Sir2 (Cat. No. S5313) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1,3. Antibody 1 μg/mL 2,4. Antibody 1 μg/mL + Sir2 immunizing peptide (human, 722-737)
Immunoblotting Nuclear extract of NIH-3T3 cells were separated on SDS-PAGE, blotted with Anti-Sir2 (Cat. No. S5313) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 0.25 μg/mL 2. Antibody 0.5 μg/mL 3. Negative control: without first antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~110 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:50 using mouse NIH3T3 cells |
| microarray: suitable | |
| western blot: 1:2,000 using mouse NIH/3T3 cells | |
| UniProt accession no. | Q923E4  |
| Application: | Anti-Sir2 (AS-16) antibody produced in rabbit has been used in • western blotting • immunoprecipitation • antibody microarray |
| Biochem/physiol Actions: | Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide. |
| Biochem/physiol Actions: | Sir2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). Sir2 protein is an NAD-dependent histone deacetylase. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity. |
| Immunogen: | synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |