Anti-Sirt1 (C-terminal) antibody produced in rabbit
SIGMA/S5322 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-S. cerevisiae, HOMOLOG-LIKE 1; Anti-SIR2; Anti-SIR2α; Anti-SIR2L1; Anti-Sirtuin 1
MDL Number: MFCD08705602
Product Type: Chemical
Immunofluorescence U-2-OS cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Rabbit Anti-SIRT1 (C-terminal) (Cat. No. S5322). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting Whole extract of Jurkat cells was separated on SDS-PAGE and probed with Rabbit Anti-SIRT1 (C-terminal) (Cat. No. S5322). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 5 μg/mL antibody 3. 5 μg/mL antibody + SIRT1 immunizing peptide (human, 723-736)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~120 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using extracts of Jurkat cells |
| indirect immunofluorescence: 10-20 μg/mL using human U-2-OS cells | |
| western blot: 1-2 μg/mL using whole extracts of Jurkat cells | |
| UniProt accession no. | Q96EB6  |
| Application: | Anti-Sirt1 (C-terminal) antibody has been used in • immunoblotting • immunoprecipitation • immunofluorescence • immunohistochemistry • western blotting |
| Biochem/physiol Actions: | SIRT1 (sirtuin 1) functions as a regulator of various metabolic pathways, and influences the pathophysiology of several metabolic diseases. It is a regulator of protein deacetylation, and is a candidate therapeutic target in non-alcoholic fatty liver disease (NAFLD), amyotrophic lateral sclerosis (ALS), kidney disease, and pulmonary disease. It also participates in tumorigenesis, and whether it functions as an oncogene or as a tumor suppressor depends upon the tumor type. In ancreatic ductal adenocarcinoma (PDAC) its elevated expression is linked with poor prognosis, and innon-small-cell lung cancer (NSCLC) it suppresses the expression of tumor suppressor p27. It is also thought to function as a suppressor of cardiovascular disorders, such as myocardial infarction, or neurodegenerative diseases, such as Alzheimer′s disease (AD) or Parkinson′s disorder (PD). |
| Biochem/physiol Actions: | Sirtuin-1 (SIRT1) is a NAD-dependent deacetylase which has been implicated in the regulation of several transcription factors, including p53, forkhead box protein O (FOXO), nuclear factor kappa-B (NFκB), myocyte-specific enhancer factor 2 (Mef2), and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SIRT1 (sirtuin 1) belongs to the silent information regulator (SIR) 2 family and is a class to HDAC (histone deacetylase). It is expressed in various tissues including brain, liver, pancreas, adipose tissue, and skeletal muscle. SIRT1 is also known as the longevity gene. |
| General description: | Sirtuin-1 (SIRT1) resides in the nucleus. |
| Immunogen: | synthetic peptide corresponding to amino acids 723-736 of human SIRT1, conjugated to KLH via an N-terminal cysteine residue. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |