Anti-SUMO-1 (C-terminal) antibody produced in rabbit
SIGMA/S5446 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GMP1; Anti-PIC1; Anti-SMT3C; Anti-SMT3H3; Anti-Sentrin-1; Anti-Small Ubiquitin-related modifier-1; Anti-UBL1
MDL Number: MFCD02096449
Product Type: Chemical
Immunoblotting
Nuclear fraction of HeLa cell lysate (Fig. A) and SUMO-1 recombinant proteins (50 μg/Lane) (Fig. B) were separated on SDS-PAGE, blotted with Rabbit Anti-SUMO-1 (C-terminal) (Cat. No. S5446) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Sumoylated nuclear proteins are detected at 90-110 kDa.
Figure A — Nuclear fraction of HeLa cells lysate: (Lane 1) Antibody 1:1,000; (Lane 2) Antibody 1:1,000 + Inhibition with SUMO-1 immunizing peptide (human 86-97) (20 μg/mL).
Figure B — Recombinant SUMO-1: (Lane 3) SUMO-1, Human with Antibody 1:1,000 + Inhibition with SUMO-1 immunizing peptide (human 86-97) (20 μg/mL); (Lane 4:) SUMO-1, Human with Antibody 1:1,000; (Lane 5) SUMO-1-GST with Antibody 1:1,000.
Nuclear fraction of HeLa cell lysate (Fig. A) and SUMO-1 recombinant proteins (50 μg/Lane) (Fig. B) were separated on SDS-PAGE, blotted with Rabbit Anti-SUMO-1 (C-terminal) (Cat. No. S5446) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Sumoylated nuclear proteins are detected at 90-110 kDa.
Figure A — Nuclear fraction of HeLa cells lysate: (Lane 1) Antibody 1:1,000; (Lane 2) Antibody 1:1,000 + Inhibition with SUMO-1 immunizing peptide (human 86-97) (20 μg/mL).
Figure B — Recombinant SUMO-1: (Lane 3) SUMO-1, Human with Antibody 1:1,000 + Inhibition with SUMO-1 immunizing peptide (human 86-97) (20 μg/mL); (Lane 4:) SUMO-1, Human with Antibody 1:1,000; (Lane 5) SUMO-1-GST with Antibody 1:1,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 14 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1-2 μg/mL using nuclear extract of the human epitheloid carcinoma HeLa cell line | |
| UniProt accession no. | P63165  |
| Application: | Anti-SUMO-1 (C-terminal) antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | Anti-SUMO-1 (C-terminal) recognizes unconjugated SUMO-1 (14 kDa), SUMO-1-fusion protein, as well as proteins covalently conjugated to SUMO-1, eg. RanGAP1, 90kDa |
| Biochem/physiol Actions: | Small ubiquitin-related modifier 1 (SUMO-1) is covalently conjugated to the lysine side in proteins through its C-terminal glycine residue by an isopeptide bond. Their conjugation with cellular proteins has been correlated wide variety of biological processes including inflammation, oncogenesis nuclear transport, cell cycle control and viral infection response. SUMO-1 serves as a substrate for Ran GTPase-activating protein (RanGAP1). Unmodified RanGAP1 is present in the cytoplasm, suggesting that modification by SUMO-1 may target RanGAP1 to the nuclear pore complex (NPC). Haploinsufficiency in the SUMO-1 gene is implicated in the Cleft Lip and Palate. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Small ubiquitin-related modifier 1 (SUMO-1) is a highly conserved small ubiquitin-related modifier, also known as sentrin. SUMO-1 gene is mapped to human chromosome 2q33.1. |
| Immunogen: | synthetic peptide corresponding to amino acids 86-97 located at the C-terminus of human SUMO-1, conjugated to KLH. This sequence is identical in many species including rat, mouse, bovine, chicken, and Xenopus, and highly conserved (single amino acid substitution) in C. elegans SMT3. The sequence has only 66% homology to human SUMO-2 and SUMO-3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |