Anti-Serotonin antibody produced in rabbit
SIGMA/S5545 - whole antiserum
Synonym: Anti-Serotonin; Rabbit Anti-Serotonin; Serotonin Detection Antibody
MDL Number: MFCD00162839
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Neuroanatomy of the vestimentiferan tubeworm Lamellibrachia satsuma provides insights into the evolution of the polychaete nervous system. By: Miyamoto, N., Shinozaki, A., et al. in PLoS One, 2013. PubMed ID: 23372830 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0055151), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Neuroanatomy of the vestimentiferan tubeworm Lamellibrachia satsuma provides insights into the evolution of the polychaete nervous system. By: Miyamoto, N., Shinozaki, A., et al. in PLoS One, 2013. PubMed ID: 23372830 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0055151), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Neuroanatomy of the vestimentiferan tubeworm Lamellibrachia satsuma provides insights into the evolution of the polychaete nervous system. By: Miyamoto, N., Shinozaki, A., et al. in PLoS One, 2013. PubMed ID: 23372830 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0055151), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Rat appendix sections with 1:8,000 Rabbit Anti-Serotonin (Cat. No. S5545) using biotin/ExtrAvidin®-Peroxidase.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:8,000 using sections of normal human appendix or serotonin-containing carcinoid tumors |
| immunohistochemistry (frozen sections): 1:5,000 using perfusion-fixed (4% paraformaldehyde), frozen sections of rat brain. |
| Application: | Anti-Serotonin antibody has been used in immunocytochemistry. |
| Biochem/physiol Actions: | Serotonin/5-HT, the midbrain raphe system controls mood, emotion, cognition and motor functions, circadian and neuroendocrine rhythms, like food intake, sleep and reproductive activity. It participates in natural reward-related physiology and behaviour, from feeding to sexual activity. |
| Biochem/physiol Actions: | The antibody reacts with serotonin-containing fibers in paraformaldehyde perfusion-fixed, frozen sections of rat brain. The product specifically stains enterochromaffin cells in formalin-fixed, paraffin embedded sections of normal human appendix and serotonin-containing carcinoid tumors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Serotonin/5-HT (5-hydroxytryptamine) is a neurotransmitters present in the brain. Anti-Serotonin specifically stains enterochromaffin cells in formalin-fixed, paraffin-embedded sections of normal human appendix and serotonin-containing carcinoid tumors. In the central nervous system (CNS), the antiserum reacts with serotonin-containing fibers in perfusion-fixed, free-floating sections of rat brain. |
| Immunogen: | Serotonin creatinine sulfate complex conjugated with formaldehyde to BSA. |
| Physical form: | Supplied as a liquid containing 15 mM sodium azide as preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |