Anti-α-Synuclein antibody, Mouse monoclonal
SIGMA/S5566 - clone Syn211, purified from hybridoma cell culture
Synonym: Anti-SNCA
MDL Number: MFCD02095740
Product Type: Chemical
Immunoblotting Recombinant human α-Synuclein extract (Cat. No. S7820) was separated on SDS-PAGE and probed with 0.25 μg/mL Monoclonal Anti-α-Synuclein Clone: Syn211 (Cat. No. S5566). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.03 μg recombinant human α-Synuclein extract 2. 0.06 μg recombinant human α-Synuclein extract 3. 0.125 μg recombinant human α-Synuclein extract
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Syn211, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 19 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | rat, mouse |
| species reactivity | zebra finch, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 0.25 μg/mL using recombinant human α·-synuclein. | |
| UniProt accession no. | P37840  |
| Application: | Anti-α-Synuclein antibody, Mouse monoclonal has been used in western blotting and ELISA. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Flow cytometry/Cell sorting (1 paper) Immunocytochemistry (1 paper) |
| Biochem/physiol Actions: | Accumulation of α-synuclein in oligodendrocytes results in the loss of myelin and causes neurodegeneration, leading to multiple system atrophy. Synuclein-α is known to induce oligodendrocyte maturation. The encoded protein is considered to be an important aggregate of Lewy bodies, contributing to the pathogenesis of Parkinson disease. |
| Biochem/physiol Actions: | Synuclein-α is known to induce oligodendrocyte maturation. Accumulation of α-synuclein in oligodendrocytes results in the loss of myelin and causes neurodegeneration, leading to multiple system atrophy. Mutations in the SNCA gene results in Parkinson’s disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The SNCA (synuclein α) gene codes for a 140-amino acid protein α-synuclein, that is mapped to human chromosome 4q21-23. |
| General description: | The SNCA (synuclein α) gene is mapped to human chromosome 4q22.1. The gene encodes a small synaptic protein called α-synuclein. |
| Immunogen: | recombinant human α synuclein. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1 % bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |