Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in rabbit
SIGMA/S5569 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-IPO-B; Anti-
MDL Number: MFCD06798546
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluoresence. NRK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 10 μg/mL Anti-Superoxide Dismutase (MnSOD) (DD-17) antibody produced in Rabbit (Cat. No. S5069) (A). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 1:500 Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in Rabbit (Cat. No. S5569) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody. Results Two Anti Superoxide Dismutase (MnSOD) antibodies, S5069 (A) and S5569 (B) target different regions of Superoxide Dismutase (MnSOD) show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence Rat NRK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:250 Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in Rabbit (Cat. No. S5569) followed by 1:500 Anti-Rabbit IgG-Cy3 antibody.
Western Blotting HeLa mitochondria extract were separated on SDS-PAGE and probed with Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in Rabbit (Cat. No. S5569). The antibody was developed using 1:30,000 Peroxidase-Anti-Rabbit IgG. Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:4,000 antibody 4. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~24 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:250-1:500 using methanol-acetone-fixed rat NRK cells |
| western blot: 1:1,000-1:2,000 using whole extract of rat brain and a chemiluminescent detection reagent | |
| western blot: 1:2,000-1:4,000 using human HeLa mitochondria extract and a chemiluminescent detection reagent | |
| UniProt accession no. | P04179  |
| Application: | Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in rabbit may be used in immunoblotting and indirect immunofluorescence. |
| Biochem/physiol Actions: | Anti-Superoxide Dismutase (MnSOD) (KC-19) recognizes human and rat Superoxide Dismutase (MnSOD). |
| Biochem/physiol Actions: | Manganese superoxide dismutase (MnSOD) is essential for the survival of aerobic life. This protein is inducible by tumor necrosis factor (TNF) and protects cells from TNF-mediated apoptosis. MnSOD is proposed to function as a tumor suppressor by modulating the activity of redox-sensitive transcription factors and specific signal mediators. Overexpression of the MnSOD gene protects neurons from N-methyl-D-aspartate receptor (NMDA) and nitric oxide-induced neurotoxicity. A decline in the MnSOD activity occurs in aging, progeria, cancer, asthma and transplant rejection. |
| Biochem/physiol Actions: | Superoxide Dismutase 2 (SOD2) is an antioxidant enzyme and catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen. The enzyme offers protection against oxidative damage induced by reactive oxygen species and also is radioprotective. The gene is associated with the metastasis and invasion process of salivary adenoid cystic carcinoma. Manganese SOD is involved in detoxification. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Manganese superoxide dismutase (MnSOD) protein is a homotetrameric SOD. This protein is composed of four subunits, each containing one Mn2+. MnSOD enzyme is synthesized in the cytosol and is modified for its transport into the mitochondria where it resides in the matrix. |
| General description: | The SOD2 (superoxide dismutase 2) gene is mapped to human chromosome 6q25.3. The encoded protein functions as a tetramer and is localized to mitochondria. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 202-220 of human superoxide dismutase (MnSOD), conjugated to KLH. The corresponding sequence is identical in apes and monkeys, differs by one amino acid in mouse and horse, by two amino acids in cow, and by four amino acids in rat. |
| Physical form: | Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |