Sodium bicarbonate
SIGMA/S5761 - powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture
Synonym: Sodium hydrogen carbonate; soda bicarbonate
CAS Number: 144-55-8
Empirical Formula (Hill Notation): CHNaO3
Molecular Weight: 84.01
EC Number: 205-633-8
MDL Number: MFCD00003528
Linear Formula: NaHCO3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S5761-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S5761-1KG
| assay | 99.5-100.5% |
| density | 2.16 g/mL at 25 °C (lit.) |
| form | powder |
| grade | Molecular Biology |
| InChI | 1S/CH2O3.Na/c2-1(3)4;/h(H |
| InChI key | UIIMBOGNXHQVGW-UHFFFAOYSA |
| pH | 6.8-7.4 |
| pKa (25 °C) | (1) 6.37, (2) 10.25 (carbonic acid) |
| product line | BioReagent |
| quality | 300 |
| Quality Level | 300  |
| SMILES string | [Na+].OC([O-])=O |
| solubility | water: soluble 100 mg/mL |
| technique(s) | cell culture | insect: suitable |
| cell culture | mammalian: suitable |
| Application: | Sodium bicarbonate has been used: • as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC) • as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells • as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells • as a component of medium used for cell culture of human embryonic kidney cells • as a component of Beltsville thawing solution used for the dilution of semen |
| General description: | Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 5 kg in poly drum |
| Packaging: | 500 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 99.5-100.5% |
| Density | 2.16 g/mL at 25 °C (lit.) |
| UNSPSC | 12161702 |