Monoclonal Anti-Synaptophysin antibody produced in mouse
SIGMA/S5768 - clone SVP-38, ascites fluid
Synonym: Synaptophysin Antibody; Synaptophysin Antibody - Monoclonal Anti-Synaptophysin antibody produced in mouse
MDL Number: MFCD00164741
Product Type: Chemical
Western Blotting JOURNAL CITATION: Mover is a homomeric phospho-protein present on synaptic vesicles. By: Ahmed, S., Wittenmayer, N., et al. in PLoS One, 2013. PubMed ID: 23723986 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0063474), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Mover is a homomeric phospho-protein present on synaptic vesicles. By: Ahmed, S., Wittenmayer, N., et al. in PLoS One, 2013. PubMed ID: 23723986 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0063474), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of frozen acetone fixed Rat cerebellum sections with 1:200 Monoclonal Anti-Synaptophysin, Clone: SVP-38 (Cat. No. S5768) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:200 Monoclonal Anti-Synaptophysin, Clone: SVP-38 (Cat. No. S5768). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Rat brain 2. PC-12 3. Mouse brain
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SVP-38, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 38 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | pig, rat, human, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunohistochemistry (frozen sections): 1:200 using rat cerebellum | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P08247  |
| Application: | Monoclonal Anti-Synaptophysin antibody has been used in western blotting. |
| Biochem/physiol Actions: | Labels synaptophysin in neurons, neuromuscular junctions, benign and malignant neural tumors, paraganglia cells, hypophysis, pancreatic islet cells, and adrenal cells. |
| Biochem/physiol Actions: | Synaptophysin participates in calcium dependent exocytosis. The antibody detects synaptophysin in many types of benign and malignant neural and epithelial neuroendocrine neoplasms. It is widely used as a potential marker to study the circulation of synapses in the brain and to discover the basic features of the life cycle of synaptic vesicles (SVs). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Synaptophysin (SYP) is an integral membrane glycoprotein of 38,000 Da and it has four membrane-spanning domains. Synaptophysin is localized to neurons, neuromuscular junctions, paraganglia cells, hypophysis, pancreatic islet cells, and adrenal cells. SYP gene is mapped to human chromosome Xp11.23. Monoclonal Anti-Synaptophysin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | Rat retina synaptosome. |
| Physical form: | Monoclonal Anti-Synaptophysin is provided as ascites fluid containing 0.1% sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |