Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse
SIGMA/S5816 - SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym: SDF-1α/PBSF
MDL Number: MFCD02100631
Product Type: Chemical
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| assay | >97% (SDS-PAGE) |
| biological source | mouse |
| form | powder |
| impurities | <1 EU/μgtested (LAL test) |
| mol wt | predicted mol wt ~8 kDa |
| packaging | pkg of 10 μg |
| potency | 0.15-0.6 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P40224  |
| Analysis Note: | The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4. |
| Application: | Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles. |
| Biochem/physiol Actions: | SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α.. |
| Biochem/physiol Actions: | Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis. |
| General description: | Stromal derived factor-1 (SDF-1) is localized to adipocytes. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin. |
| Preparation Note: | Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |