Sephadex® G-50
SIGMA/S5897 - BioReagent, DNA grade, Molecular Biology, fine
CAS Number: 9004-54-0
MDL Number: MFCD23701222.obs1210407
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S5897-25G
| bead diameter | 20-80 μm |
| foreign activity | DNase, RNase, none detected |
| form | beads |
| grade | Molecular Biology |
| InChI | 1S/C18H32O16/c19-1-5(21)9 |
| InChI key | FZWBNHMXJMCXLU-UHFFFAOYSA |
| matrix | cross-linked dextran |
| pH | 2-10 |
| pore size | exclusion limit (20 bp dsDNA (rA)20) |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | O1C(C(C(C(C1CO)O)O)O)OCC2 |
| storage temp. | room temp |
| swelling | 1 g swells to 9-11 mL |
| technique(s) | DNA purification: suitable |
| Application: | Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality. |
| Features and Benefits: | • Excellent recovery • Minimal sample dilution • Quick and easy to use |
| General description: | Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances. S5897-100G′s updated product number is GE17-0573-02 |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Packaging: | 25 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 23151817 |