Anti-STAT3 antibody produced in rabbit
SIGMA/S5933 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD01092492
Product Type: Chemical
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| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 92 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 10 μg/mL using A431 cells |
| immunoprecipitation (IP): 4 μg using STAT3 from 0.5–1 mg of an EGF stimulated human A431 cell lysate | |
| western blot (chemiluminescent): 0.5-2 μg/mL using lysates from EGF stimulated human A431, mouse WEHI and rat L6 cells | |
| UniProt accession no. | P40763  |
| Application: | Anti-STAT3 antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Signal transducer and activator of transcription 3 (STAT3) signaling participates in cancer inflammation. This protein interacts with nuclear factor-κB (NF-κB). STAT3 regulates the function of several genes that take part in proliferation, apoptosis, metastasis, angiogenesis, immune response, and cell differentiation. Overexpression of the STAT3 gene is associated with lymphomas, prostate carcinoma, breast cancer, hepatocellular carcinoma, pancreatic adenocarcinoma, cholangiocarcinoma, and ovarian carcinoma. |
| Biochem/physiol Actions: | The antibody is specific for STAT3. It may detect an additional 66 kDa band using high concentrations or long exposures. The sequence recognized by the antibody (688-722) is identical in mouse and rat STAT3. The first 28 amino acids are identical to mouse STAT3B. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Signal transducer and activator of transcription 3 (STAT3) protein is a member of the STAT family of proteins. It consists of a DNA binding domain, a Src homology-2 (SH-2) domain, an N-terminal coiled-coiled domain, and a C-terminal transactivation domain. The STAT3 gene is located on the human chromosome at 17q21.2. |
| Immunogen: | GST fusion protein corresponding to amino acids 688-722 of human STAT3, expressed in bacteria. |
| Physical form: | Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |