Anti-Synphilin-1 antibody produced in rabbit
SIGMA/S5946 - ~2 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-SNCAIP; Anti-Synuclein-α-interacting protein
MDL Number: MFCD02264134
Product Type: Chemical
Whole cell lysates were separated on SDS-PAGE and blotted with Anti-Synphilin-1 (Cat. No. S5946). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Additional bands observed around 75 kDa may represent alternatively spliced synphilin-1 isoforms. Antibody dilution 1:2,000 Lanes 1: HEK293 cells (untransfected) 2,3: HEK293 cells transfected with human synphilin-1 3: Inhibition of antibody binding with synphilin-1 immunizing peptide (human 829-847) (20 μg/mL)
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Synphilin-1 antibody produced in Rabbit (Cat. No. S5946) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Synphilin-1 (Lane 2) while no staining is detected in the wild type sample-HEK-293T (Lane 1). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:2,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 130 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.25-0.5 μg/mL using HEK-293 cells expressing human synphilin-1 |
| UniProt accession no. | Q9Y6H5  |
| Application: | Anti-Synphilin-1 antibody produced in rabbit has been used in co-immunoprecipitation and western blotting. |
| Biochem/physiol Actions: | Synphilin-1 associates with and is ubiquitinated by several proteins, including α -synuclein, parkin, dorfin and siah E3 ubiquitin protein ligase 1 (SIAH1), and is a major component of Lewy bodies in Parkinson′s disease (PD). It is associated with synaptic vesicles and is modulated by α-synuclein. The central domain of synphilin-1 has been suggested to be required for the formation of aggregates and cytotoxicity. Synphilin-1 association with α -synuclein promotes the formation of cytosolic inclusions. Mutation of synphilin-1 in PD patients at amino acid R621C, is implicated in these sequestration, ubiquitination and proteasomal inhibition leading to increased accumulation of toxic intermediates. Cells with this mutation are more prone to staurosporine-induced cell death highlighting its cytoprotective functionality. In human postmortem brain tissue, synphilin-1, like a-synuclein is present in neurophil. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Synphilin-1 (synuclein a-interacting protein 1, SNCAIP), is a cytoplasmic protein that interacts with α-synuclein in neurons. Synphilin-1 contains several protein-protein interaction domains, including six ankyrin-like repeats, coiled-coil domain, and an ATP/GTP binding domain. The synphilin gene produces at least nine transcript variants encoding seven distinct proteins. Synphilin transcripts are widely expressed in many tissues with highest levels found in the brain, heart and placenta. Synphilin-1 is highly enriched in presynaptic nerve terminals. |
| Immunogen: | synthetic peptide corresponding to amino acids 829-847 of human synphilin, conjugated to KLH. This sequence is identical in dog synphilin-1 and highly conserved (84% identity) in mouse, rat, and bovine synphilin-1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |