Anti-Sirt7 antibody produced in rabbit
SIGMA/S5947 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-SIR2L7; Anti-Sir2-related protein type 7; Anti-Sirtuin (silent mating type information regulation 2 homolog) 7
MDL Number: MFCD07784768
Product Type: Chemical
Immunofluorescence (Indirect) Human HEK-293T cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton-X-100. Cells were stained with 2.5 μg/mL Anti-SIRT7 (Cat. No. S5947) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate (A), or stained with DAPI (B).
Immunoprecipitation Rabbit Anti-SIRT7 antibody (Cat. No. S5947) was used to immunoprecipitate SIRT7 from HEK-293T cells expressing recombinant human SIRT7-V5. Detection antibody: Monoclonal Anti-V5 antibody, clone V5-10 (Cat. No. V8012). Lanes 1: 2 μg Antibody 2: 4 μg Antibody 3: Negative control (without primary antibody)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~45 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse (predicted), human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-4 μg using extracts of HEK-293T cells expressing human Sirt7 |
| indirect immunofluorescence: 2-4 μg/mL using human HEK-293T cells | |
| western blot: 1-2 μg/mL using whole extracts of HEK-293T cells expressing human Sirt7 | |
| UniProt accession no. | Q9NRC8  |
| Application: | Anti-Sirt7 antibody produced in rabbit has been used in western blotting analysis and immunofluorescence detection. |
| Biochem/physiol Actions: | Sirt7 (sirtuin 7) is extensively found in protein associated with active rRNA genes (rDNA), in the nucleolus. It interacts with RNA polymerase I (Pol I) and histones. Overexpression of Sirt7 increases Pol I-mediated transcription, whereas knockdown of Sirt7 or inhibition of its catalytic activity results in decreased association of Pol I with rDNA and reduces Pol I transcription. Depletion of Sirt7 stops cell proliferation and triggers apoptosis. High levels of Sirt7 expression are associated with breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase (Sir2) is one of the silent information regulator genes in yeast. It belongs to a family of proteins, that is found in organisms ranging from bacteria to complex eukaryotes. Proteins of this family share a core domain showing 25-60% sequence identity. The mammalian Sir2 gene family is comprised of seven members which are designated as Sirt1-7. |
| Immunogen: | synthetic peptide corresponding to amino acids 35-51 of human Sirt7, conjugated to KLH via a C-terminal cysteine residue. The sequence is identical in mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |