Sephadex® G-50
SIGMA/S6022 - BioReagent, DNA grade, Molecular Biology, medium
CAS Number: 9004-54-0
MDL Number: MFCD23701222.obs1210407
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S6022-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S6022-25G
| bead diameter | 50-150 μm |
| foreign activity | DNase, RNase, none detected |
| form | beads |
| grade | Molecular Biology |
| InChI | 1S/C18H32O16/c19-1-5(21)9 |
| InChI key | FZWBNHMXJMCXLU-UHFFFAOYSA |
| pH | 2-10 |
| pore size | exclusion limit (20 bp dsDNA; (rA)20) |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | O1C(C(C(C(C1CO)O)O)O)OCC2 |
| storage temp. | room temp |
| swelling | 1 g swells to 9-11 mL |
| Application: | Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions. |
| Application: | Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions. |
| Features and Benefits: | • Excellent recovery • Minimal sample dilution • Quick and easy to use |
| General description: | Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required. |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Packaging: | 25, 100 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 23151817 |